验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 4 publications below |
| WB | See 7 publications below |
产品信息
11127-2-AP targets SH3BP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | SH3BP5 fusion protein Ag1592 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SH3-domain binding protein 5 (BTK-associated) |
| Synonyms | SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 32 kDa, 50-83 kDa |
| GenBank Accession Number | BC010123 |
| Gene Symbol | SH3BP5 |
| Gene ID (NCBI) | 9467 |
| RRID | AB_2189373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60239 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3BP5 antibody 11127-2-AP | Download protocol |
| IHC protocol for SH3BP5 antibody 11127-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Hepatology Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury. | ||
J Hepatol Sab (Sh3bp5) dependence of JNK mediated inhibition of mitochondrial respiration in palmitic acid induced hepatocyte lipotoxicity.
| ||
Cell Death Dis JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.
| ||
Front Pharmacol Scoparone Improves Nonalcoholic Steatohepatitis Through Alleviating JNK/Sab Signaling Pathway-Mediated Mitochondrial Dysfunction.
| ||
Biomolecules Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.
| ||
Biomed Pharmacother Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway |