验证数据展示
经过测试的应用
Positive WB detected in | C6 cells, mouse brain, rat brain |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | Endophilin 1 fusion protein Ag3114 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SH3-domain GRB2-like 2 |
别名 | CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4 |
计算分子量 | 39.8 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC032825 |
基因名称 | Endophilin 1 |
Gene ID (NCBI) | 6456 |
RRID | AB_2187398 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99962 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
IHC protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
IP protocol for Endophilin 1 antibody 12435-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Neoplasia Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.
| ||
Invest Ophthalmol Vis Sci Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma |