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  • Featured Product
  • KD/KO Validated

Endophilin 1 Polyclonal antibody

Endophilin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12435-1-AP

Print datasheet

Synonyms

CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4

验证数据展示

  • WB analysis using 12435-1-AP

    WB analysis using 12435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12435-1-AP (SH3GL2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12435-1-AP

    IP experiment of mouse brain using 12435-1-AP

    IP result of anti-Endophilin 1 (IP:12435-1-AP, 4ug; Detection:12435-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human gliomas using 12435-1-AP

    IHC staining of human gliomas using 12435-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12435-1-AP (SH3GL2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12435-1-AP

    IHC staining of human gliomas using 12435-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12435-1-AP (SH3GL2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inC6 cells, mouse brain, rat brain
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12435-1-AP targets Endophilin 1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Endophilin 1 fusion protein Ag3114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3-domain GRB2-like 2
Synonyms CNSA2, EEN B1, Endophilin 1, Endophilin A1, SH3 domain GRB2 like 2, SH3 domain protein 2A, SH3D2A, SH3GL2, SH3P4
Calculated Molecular Weight 39.8 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC032825
Gene Symbol Endophilin 1
Gene ID (NCBI) 6456
RRIDAB_2187398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IHC protocol for Endophilin 1 antibody 12435-1-APDownload protocol
IP protocol for Endophilin 1 antibody 12435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Neoplasia

Loss of Sh3gl2/Endophilin A1 Is a Common Event in Urothelial Carcinoma that Promotes Malignant Behavior.

Authors - Majumdar Shyama S
  • KD Validated
View Article
humanWB

Invest Ophthalmol Vis Sci

Bioinformatics Analysis and Experimental Identification of Immune-Related Genes and Immune Cells in the Progression of Retinoblastoma

Authors - Shuilian Chen
View Article