Bif-1 Polyclonal antibody

Bif-1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15422-1-AP

Print datasheet

Synonyms

Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1



经过测试的应用

Positive WB detected inmouse kidney tissue, HEK-293 cells, human placenta tissue, mouse heart tissue, mouse placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Bif-1 Ready-To-Use IHC Kit. Bif-1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15422-1-AP targets Bif-1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications ChIP, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Bif-1 fusion protein Ag7710 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3-domain GRB2-like endophilin B1
Synonyms Bax interacting factor 1, Bif 1, Bif1, Endophilin B1, SH3GLB1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC007455
Gene Symbol SH3GLB1
Gene ID (NCBI) 51100
RRIDAB_2254564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Bif-1 antibody 15422-1-APDownload protocol
IHC protocol for Bif-1 antibody 15422-1-APDownload protocol
IF protocol for Bif-1 antibody 15422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

Eur J Pharmacol

SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.

Authors - Anbo Gao
humanWB

J Exp Clin Cancer Res

SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.

Authors - Chia-Hung Chien
mouseWB

Oncotarget

Autophagy-related gene expression is an independent prognostic indicator of glioma.

Authors - Huixue Zhang
humanWB,ChIP

BMC Cancer

Role of SH3GLB1 in the regulation of CD133 expression in GBM cells

Authors - Chia-Hung Chien