验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human testis tissue, mouse lung tissue, mouse testis tissue, rat lung tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SH3GLB2 Ready-To-Use IHC Kit. SH3GLB2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15897-1-AP targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SH3GLB2 fusion protein Ag8712 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SH3-domain GRB2-like endophilin B2 |
| 别名 | PP9455, PP6569, PP578, KIAA1848, Endophilin-B2 |
| 计算分子量 | 395 aa, 44 kDa |
| 观测分子量 | 47-50 kDa |
| GenBank蛋白编号 | BC014635 |
| 基因名称 | SH3GLB2 |
| Gene ID (NCBI) | 56904 |
| RRID | AB_2187269 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NR46 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3GLB2 antibody 15897-1-AP | Download protocol |
| IHC protocol for SH3GLB2 antibody 15897-1-AP | Download protocol |
| IF protocol for SH3GLB2 antibody 15897-1-AP | Download protocol |
| IP protocol for SH3GLB2 antibody 15897-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain Pathol Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1. | ||
J Biol Chem Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.
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