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SHANK2 Polyclonal antibody

SHANK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 27272-1-AP

Print datasheet

Synonyms

SHANK, ProSAP1, KIAA1022, CTTNBP1, CORTBP1

验证数据展示

  • IP experiment of mouse brain using 27272-1-AP

    IP experiment of mouse brain using 27272-1-AP

    IP result of anti-SHANK2 (IP:27272-1-AP, 4ug; Detection:27272-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

  • WB analysis using 27272-1-AP

    WB analysis using 27272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27272-1-AP (SHANK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27272-1-AP targets SHANK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SHANK2 fusion protein Ag26101 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 and multiple ankyrin repeat domains 2
Synonyms SHANK, ProSAP1, KIAA1022, CTTNBP1, CORTBP1
Calculated Molecular Weight 159 kDa
Observed Molecular Weight 158-200 kDa
GenBank Accession NumberNM_012309
Gene Symbol SHANK2
Gene ID (NCBI) 22941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3 and multiple ankyrin repeat domains protein 2 (SHANK2) is also named as CORTBP1, KIAA1022, PROSAP1. SHANK2 code a scaffolding protein located at the postsynaptic membrane of glutamatergic neurons (PMID: 32987185). SHANK2 encodes for a postsynaptic scaffolding protein at glutamatergic synapses in the brain, essential for proper synapse formation, development and plasticity (PMID: 11283303, PMID: 12065602). As SHANK2 directly interacts with IRSp53 (insulin receptor substrate p53), it may be involved in insulin signaling in the brain, making this pathway susceptible to SHANK2 mutations (PMID: 33483523). In the SFARI gene database, SHANK2 is categorized as a high confidence autism risk gene. In addition, SHANK2 has been linked to the pathology of neuropsychiatric (schizophrenia, bipolar disorder) and neurodegenerative disorders (PMID: 33483523).

实验方案

Product Specific Protocols
WB protocol for SHANK2 antibody 27272-1-APDownload protocol
IP protocol for SHANK2 antibody 27272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols