验证数据展示
经过测试的应用
| Positive WB detected in | human plasma |
| Positive IHC detected in | mouse testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
18200-1-AP targets SHBG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | goat |
| 免疫原 | SHBG fusion protein Ag12683 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sex hormone-binding globulin |
| 别名 | Testosterone-estradiol-binding globulin, Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin |
| 计算分子量 | 402 aa, 44 kDa |
| 观测分子量 | 42-44 kDa |
| GenBank蛋白编号 | BC101785 |
| 基因名称 | SHBG |
| Gene ID (NCBI) | 6462 |
| RRID | AB_2878517 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04278 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHBG antibody 18200-1-AP | Download protocol |
| IHC protocol for SHBG antibody 18200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. | ||
Biol Reprod Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. | ||
Theriogenology FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway |