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验证数据展示

  • WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

    WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

    IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

    IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of human pancreas cancer using 20697-1-AP (same clone as 20697-1-PBS)

    IHC staining of human pancreas cancer using 20697-1-AP (same clone as 20697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)

    IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of mouse embryo using 20697-1-AP (same clone as 20697-1-PBS)

    IHC staining of mouse embryo using 20697-1-AP (same clone as 20697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SHH antibody included
  • IF Staining of mouse kidney using 20697-1-AP (same clone as 20697-1-PBS)

    IF Staining of mouse kidney using 20697-1-AP (same clone as 20697-1-PBS)

    Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 20697-1-AP (same clone as 20697-1-PBS)

    IP experiment of mouse liver using 20697-1-AP (same clone as 20697-1-PBS)

    IP result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

  • WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

    WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 20697-1-AP (same clone as 20697-1-PBS)

    FC experiment of HepG2 using 20697-1-AP (same clone as 20697-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SHH Polyclonal antibody, PBS Only

SHH Polyclonal Antibody for WB, IHC, IF, FC (Intra), IP, Indirect ELISA
Cat No. 20697-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SHH Ready-To-Use IHC Kit. SHH primary antibody included.

产品信息

20697-1-PBS targets SHH in WB, IHC, IF, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sonic hedgehog homolog (Drosophila)
别名
计算分子量 50 kDa
观测分子量 45-51 kDa
GenBank蛋白编号NM_000193
基因名称 SHH
Gene ID (NCBI) 6469
RRIDAB_10694828
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15465
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival, 

and fate.


What is the molecular weight of SHH?

The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa 

N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the 

auto-processing machinery respectively (PMID: 10753901).


What is the tissue specificity of SHH?

SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development, 

SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and 

dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney, 

and lung tissue, but is not expressed in adult tissues.


What is the cellular localization of SHH?

SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface, 

whereas the C-terminal fragment diffuses from the cell.


What is the role of SHH in the Sonic hedgehog signaling pathway?

This pathway is one of the major trafficking networks that regulate key events during developmental growth 

and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects, 

tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH 

pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is 

implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178, 

11801325).