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SHH Polyclonal antibody

SHH Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 29752-1-AP

Print datasheet

Synonyms

HPE3, HLP3, HHG-1, HHG1, HHG 1

验证数据展示

  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHH antibody included
  • IHC staining of mouse embryo using 29752-1-AP

    IHC staining of mouse embryo using 29752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 29752-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SHH antibody included
  • WB analysis using 29752-1-AP

    WB analysis using 29752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29752-1-AP (SHH antibody) at dilution of 1:18000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 29752-1-AP

    FC experiment of HeLa using 29752-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHH (29752-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SHH Ready-To-Use IHC Kit. SHH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29752-1-AP targets SHH in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SHH fusion protein Ag31338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sonic hedgehog homolog (Drosophila)
Synonyms HPE3, HLP3, HHG-1, HHG1, HHG 1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight50-60 kDa
GenBank Accession NumberNM_000193
Gene Symbol SHH
Gene ID (NCBI) 6469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15465
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHH, Sonic hedgehog protein, binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes (PMID: 10753901). In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO (PMID: 10753901). SHH is synthesized as a 45 kDa precursor that undergoes an autocatalytic processing event that produces a 19 kDa N-terminal product, responsible for all signaling activities, and a 25 kDa C-terminal fragment (PMID: 10753901, PMID: 16282375).

实验方案

Product Specific Protocols
WB protocol for SHH antibody 29752-1-APDownload protocol
IHC protocol for SHH antibody 29752-1-APDownload protocol
FC protocol for SHH antibody 29752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols