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SHISA8 Polyclonal antibody

SHISA8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 30240-1-AP

Print datasheet

Synonyms

C22-CTA-250D10.9, C22orf17, LOC440829, Putative protein shisa 8, SHISA8, transmembrane protein 46 like

验证数据展示

  • IHC staining of human lung using 30240-1-AP

    IHC staining of human lung using 30240-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30240-1-AP (SHISA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung using 30240-1-AP

    IHC staining of human lung using 30240-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30240-1-AP (SHISA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30240-1-AP targets SHISA8 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human
Immunogen SHISA8 fusion protein Ag31420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane protein 46-like
Synonyms C22-CTA-250D10.9, C22orf17, LOC440829, Putative protein shisa 8, SHISA8, transmembrane protein 46 like
Calculated Molecular Weight42KD
GenBank Accession NumberNM_001353438
Gene Symbol SHISA8
Gene ID (NCBI) 440829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDB8ZZ34
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The least studied AMPAR-interacting member of the Shisa protein family is SHISA8 (CKAMP39). In HEK293 cells, SHISA8 significantly affected AMPAR-mediated current kinetics, such as current amplitude, as well as AMPAR desensitization and recovery from desensitization (PMID:33892364).

实验方案

Product Specific Protocols
IHC protocol for SHISA8 antibody 30240-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols