验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rabbit liver tissue, HepG2 cells, Jurkat cells, K-562 cells, Pig liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SHMT1 Ready-To-Use IHC Kit. SHMT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67963-1-Ig targets SHMT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rabbit samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, pig, rabbit |
| 免疫原 | SHMT1 fusion protein Ag5833 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serine hydroxymethyltransferase 1 (soluble) |
| 别名 | CSHMT, Serine methylase, SHMT, SHMT1 |
| 计算分子量 | 483 aa, 53 kDa |
| 观测分子量 | 45-53 kDa |
| GenBank蛋白编号 | BC038598 |
| 基因名称 | SHMT1 |
| Gene ID (NCBI) | 6470 |
| RRID | AB_2918714 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P34896 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHMT1 antibody 67963-1-Ig | Download protocol |
| IF protocol for SHMT1 antibody 67963-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |