验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | mouse brain tissue, human cervical cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SHMT2 Ready-To-Use IHC Kit. SHMT2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11099-1-AP targets SHMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SHMT2 fusion protein Ag1584 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine hydroxymethyltransferase 2 (mitochondrial) |
| 别名 | SHMT, EC:2.1.2.1 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC011911 |
| 基因名称 | SHMT2 |
| Gene ID (NCBI) | 6472 |
| RRID | AB_2188452 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P34897 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| IHC protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| IF protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Nat Commun Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death. | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Am J Respir Cell Mol Biol Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis. | ||
PLoS Pathog SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
| ||
Genomics SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT. |
