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  • Featured Product
  • KD/KO Validated

SHOX2 Polyclonal antibody

SHOX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16366-1-AP

Print datasheet

Synonyms

SHOT, Paired-related homeobox protein SHOT, OGI2X, OG12X, OG12

验证数据展示

  • WB analysis using 16366-1-AP

    WB analysis using 16366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16366-1-AP (SHOX2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 16366-1-AP

    IHC staining of rat brain using 16366-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16366-1-AP (SHOX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, Raji cells, PC-12 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16366-1-AP targets SHOX2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SHOX2 fusion protein Ag9156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name short stature homeobox 2
Synonyms SHOT, Paired-related homeobox protein SHOT, OGI2X, OG12X, OG12
Calculated Molecular Weight 355 aa, 38 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC008829
Gene Symbol SHOX2
Gene ID (NCBI) 6474
RRIDAB_2918046
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60902
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHOX2 antibody 16366-1-APDownload protocol
IHC protocol for SHOX2 antibody 16366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Genet

A novel hypoxia-driven gene signature that can predict the prognosis and drug resistance of gliomas

Authors - Peng Ren
  • KD Validated
View Article
mouseWB

Oxid Med Cell Longev

NRF-2/HO-1 Pathway-Mediated SHOX2 Activation Is a Key Switch for Heart Rate Acceleration by Yixin-Fumai Granules

Authors - Heng Zhang
  • KD Validated
View Article
mouseWB

Aging (Albany NY)

D-galactose causes sinoatrial node dysfunction: from phenotype to mechanism

Authors - Heng Zhang
View Article