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SHPRH Polyclonal antibody

SHPRH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21955-1-AP

Print datasheet

Synonyms

KIAA2023, SHPRH

验证数据展示

  • WB analysis of mouse testis using 21955-1-AP

    WB analysis of mouse testis using 21955-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21955-1-AP (SHPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 21955-1-AP

    IP experiment of mouse testis using 21955-1-AP

    IP result of anti-SHPRH (IP:21955-1-AP, 4ug; Detection:21955-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • IHC staining of human colon using 21955-1-AP

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 21955-1-AP

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21955-1-AP is SHPRH Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SHPRH fusion protein Ag16635 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SNF2 histone linker PHD RING helicase
Synonyms KIAA2023, SHPRH
Calculated Molecular Weight 1683 aa, 193 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC117686
Gene Symbol SHPRH
Gene ID (NCBI) 257218
RRIDAB_2878954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ149N8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHPRH antibody 21955-1-APDownload protocol
IHC protocol for SHPRH antibody 21955-1-APDownload protocol
IP protocol for SHPRH antibody 21955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols