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SIAE Polyclonal antibody

SIAE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14566-1-AP

Print datasheet

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

验证数据展示

  • WB analysis using 14566-1-AP

    WB analysis using 14566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14566-1-AP (SIAE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse testis tissue, rat heart tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14566-1-AP targets SIAE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SIAE fusion protein Ag6143 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sialic acid acetylesterase
Synonyms Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53
Calculated Molecular Weight 58 kDa
Observed Molecular Weight62-70 kDa
GenBank Accession NumberBC068450
Gene Symbol SIAE
Gene ID (NCBI) 54414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialic acids as terminal sugars of N- and O-glycosylated macromolecules are decisive for the functions of glycoconjugates both in soluble form and integrated into cell membranes .The two enzymes responsible for acetylation and de-acetylation have been described as sialate O-acetyltransferase (SOAT) and sialate O-acetylesterase (SIAE), respectively. SIAE deacetylates α(2-6)-linked sialic acid on N-linked glycans of BCR, allowing the interaction with CD22, that functions in vivo as an inhibitor of BCR signaling. (PMID: 26022516)

实验方案

Product Specific Protocols
WB protocol for SIAE antibody 14566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols