验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
13230-1-AP targets SIGLEC5 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SIGLEC5 fusion protein Ag4101 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sialic acid binding Ig-like lectin 5 |
| 别名 | CD170, CD33 antigen like 2, CD33L2, OB binding protein 2, OB BP2, OBBP2, Obesity binding protein 2, SIGLEC 5, SIGLEC5 |
| 计算分子量 | 551 aa, 61 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC029896 |
| 基因名称 | SIGLEC5 |
| Gene ID (NCBI) | 8778 |
| RRID | AB_10643385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15389 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIGLEC5 antibody 13230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner |