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验证数据展示

  • IHC staining of human placenta using 13454-1-AP

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (Siglec-6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 13454-1-AP

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (Siglec-6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Siglec-6 Polyclonal antibody

Siglec-6 Polyclonal Antibody for IHC, ELISA
Cat No. 13454-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (1)

别名

SIGLEC6, OB-BP1, CD33L1, CD33L, CD33 antigen-like 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13454-1-AP targets Siglec-6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Siglec-6 fusion protein Ag4281 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 6
别名 SIGLEC6, OB-BP1, CD33L1, CD33L, CD33 antigen-like 1
计算分子量 426 aa, 47 kDa
GenBank蛋白编号BC035359
基因名称 SIGLEC6
Gene ID (NCBI) 946
RRIDAB_2189287
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43699
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Siglec-6, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).

实验方案

Product Specific Protocols
IHC protocol for Siglec-6 antibody 13454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Adv Res

ST6GALNAC1-mediated sialylation in uterine endometrial epithelium facilitates the epithelium-embryo attachment

Authors - Xinyue Dong
View Article