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Siglec-6 Polyclonal antibody

Siglec-6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13454-1-AP

Print datasheet

Synonyms

SIGLEC6, OB-BP1, CD33L1, CD33L, CD33 antigen-like 1

验证数据展示

  • IHC staining of human placenta using 13454-1-AP

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (Siglec-6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 13454-1-AP

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (Siglec-6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13454-1-AP targets Siglec-6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Siglec-6 fusion protein Ag4281 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sialic acid binding Ig-like lectin 6
Synonyms SIGLEC6, OB-BP1, CD33L1, CD33L, CD33 antigen-like 1
Calculated Molecular Weight 426 aa, 47 kDa
GenBank Accession NumberBC035359
Gene Symbol SIGLEC6
Gene ID (NCBI) 946
RRIDAB_2189287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43699
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Siglec-6, originally identified on trophoblast cells of the placenta, is highly and consistently expressed on MCs from both mucosal and non-mucosal sites. Siglec-6 consists of 3 extracellular immunoglobulin (Ig) domains and 2 intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs), closely resembling the molecular structure of Siglec-3 (CD33) (PMID: 34289026). Siglec-6 was recruited to placental expression during human evolution, presumably to interact with sialylated ligands for specific negative signaling functions and/or to regulate leptin availability (PMID: 17580316). Siglec-6 is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptor selectively expressed by mast cells, making it a promising target for therapeutic intervention (PMID: 35406705).

实验方案

Product Specific Protocols
IHC protocol for Siglec-6 antibody 13454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Adv Res

ST6GALNAC1-mediated sialylation in uterine endometrial epithelium facilitates the epithelium-embryo attachment

Authors - Xinyue Dong
View Article