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Snf1lk Polyclonal antibody

Snf1lk Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 51045-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Sik1, Salt-inducible kinase 1, salt inducible kinase 1, Myocardial SNF1-like kinase, Msk

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1000 ug/ml

库存:现货


经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inmouse brain tissue, human liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51045-1-AP targets Snf1lk in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 Snf1lk fusion protein Ag3913 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 salt inducible kinase 1
别名 Sik1, Salt-inducible kinase 1, salt inducible kinase 1, Myocardial SNF1-like kinase, Msk
计算分子量 85 kDa
观测分子量 85-100 kDa
GenBank蛋白编号NM_010831
基因名称 Sik1
Gene ID (NCBI) 17691
RRIDAB_2187322
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP57059
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and the expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832). SIK1 also can be detected 95-100 kDa (PMID: 26567857, PMID: 22109884).

实验方案

Product Specific Protocols
WB protocol for Snf1lk antibody 51045-1-APDownload protocol
IHC protocol for Snf1lk antibody 51045-1-APDownload protocol
IF protocol for Snf1lk antibody 51045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

Phytomedicine

Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis

Authors - Daofei Song
humanWB,IHC

iScience

Demethylase FTO-mediated m6A modification of SIK1 modulates placental cytotrophoblast syncytialization in type 2 diabetes mellitus

Authors - Jie Ning
  • KD Validated
humanWB, IF

J Neuroinflammation

High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke.

Authors - Mengyan Hu
humanWB

J Cell Physiol

ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes

Authors - Xueyi Chen
mouse,ratIHC,IF

J Cereb Blood Flow Metab

Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia.

Authors - Cheng Jian J
humanWB

J Leukoc Biol

SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype.

Authors - Maria Stella Lombardi
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