- Featured Product
- KD/KO Validated
SIK1 Polyclonal antibody
SIK1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, Rat and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells |
Positive IHC detected in | mouse brain tissue, human liver tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
51045-1-AP targets SIK1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, mouse, Rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SIK1 fusion protein Ag3913 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | salt-inducible kinase 1 |
Synonyms | MSK, salt inducible kinase 1, SIK, SIK 1, SIK1, SNF1LK |
Calculated Molecular Weight | 783 aa, 85 kDa |
Observed Molecular Weight | 85-100 kDa |
GenBank Accession Number | BC038504 |
Gene Symbol | SIK1 |
Gene ID (NCBI) | 150094 |
RRID | AB_2187322 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P57059 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SIK1 antibody 51045-1-AP | Download protocol |
IHC protocol for SIK1 antibody 51045-1-AP | Download protocol |
IF protocol for SIK1 antibody 51045-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Phytomedicine Wen-Shen-Jian-Pi-Hua-Tan decoction protects against early obesity-related glomerulopathy by improving renal bile acid composition and suppressing lipogenesis, inflammation, and fibrosis | ||
J Neuroinflammation High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke. | ||
J Cell Physiol ETNPPL modulates hyperinsulinemia-induced insulin resistance through the SIK1/ROS-mediated inactivation of the PI3K/AKT signaling pathway in hepatocytes | ||
J Cereb Blood Flow Metab Role of salt-induced kinase 1 in androgen neuroprotection against cerebral ischemia. | ||
J Leukoc Biol SIK inhibition in human myeloid cells modulates TLR and IL-1R signaling and induces an anti-inflammatory phenotype. | ||
Inflamm Bowel Dis HG-9-91-01 Attenuates Murine Experimental Colitis by Promoting Interleukin-10 Production in Colonic Macrophages Through the SIK/CRTC3 Pathway. |