SIP1 Polyclonal antibody
SIP1 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, rat brain tissue |
| Positive IP detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SIP1 Ready-To-Use IHC Kit. SIP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
The immunogen of 21672-1-AP is SIP1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SIP1 fusion protein Ag16500 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | survival of motor neuron protein interacting protein 1 |
| Synonyms | Component of gems 2, Gemin 2, GEMIN2, SIP1, SIP1 delta, SMN interacting protein 1 |
| Calculated Molecular Weight | 280 aa, 32 kDa |
| Observed Molecular Weight | 32-35 kDa |
| GenBank Accession Number | BC104968 |
| Gene Symbol | SIP1 |
| Gene ID (NCBI) | 8487 |
| RRID | AB_10859249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14893 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIP1 antibody 21672-1-AP | Download protocol |
| IP protocol for SIP1 antibody 21672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. | ||
Neurobiol Dis UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins. | ||
J Mol Med (Berl) Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology. | ||
Biomolecules (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2. |
