验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25086-1-AP targets SIPA1L1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SIPA1L1 fusion protein Ag18900 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal-induced proliferation-associated 1 like 1 |
| 别名 | SIPA1 like protein 1, Signal-induced proliferation-associated 1-like protein 1, High-risk human papilloma viruses E6 oncoproteins targeted protein 1, E6TP1, E6-targeted protein 1 |
| 计算分子量 | 1803 aa, 200 kDa |
| 观测分子量 | 200 kDa |
| GenBank蛋白编号 | BC146777 |
| 基因名称 | SIPA1L1 |
| Gene ID (NCBI) | 26037 |
| RRID | AB_2714023 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43166 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
| IHC protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
| IP protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward. | ||
bioRxiv Prosapip1 in the dorsal hippocampus mediates synaptic protein composition, long-term potentiation, and spatial memory | ||
JCI Insight Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG
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