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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98101-1-RR

    FC experiment of human peripheral blood leukocytes using 98101-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98101-1-RR

    FC experiment of human peripheral blood leukocytes using 98101-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRPA Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98101-1-RR

    Affinity and Kinetic Characterization of 98101-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98101-1-RR against Human SIRP Alpha/CD172a were performed. The affinity constant is 3.39 nM.

Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody

SIRP Alpha/CD172a Uni-rAbTM Recombinant Antibody for FC
Cat No. 98101-1-RR

产品说明书

CloneNo. 240789E1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98101-1-RR targets SIRP Alpha/CD172a in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
计算分子量55 kDa
GenBank蛋白编号NM_080792
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_3672248
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78324
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

实验方案

Product Specific Protocols
FC protocol for SIRP Alpha/CD172a antibody 98101-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols