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验证数据展示

  • WB analysis of U-937 using 83729-5-RR (same clone as 83729-5-PBS)

    WB analysis of U-937 using 83729-5-RR (same clone as 83729-5-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 83729-5-RR (same clone as 83729-5-PBS)

    IF Staining of THP-1 using 83729-5-RR (same clone as 83729-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83729-5-RR

    Affinity and Kinetic Characterization of 83729-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

SIRP Alpha/CD172a Recombinant antibody, PBS Only

SIRP Alpha/CD172a Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83729-5-PBS

产品说明书

CloneNo. 240789C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83729-5-PBS targets SIRP Alpha/CD172a in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
计算分子量55 kDa
观测分子量 75-85 kDa
GenBank蛋白编号NM_080792
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP78324
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).