验证数据展示
经过测试的应用
Positive WB detected in | U-937 cells, A375 cells, PC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
产品信息
11811-1-AP targets SIRP beta 1/CD172b in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | SIRP beta 1/CD172b fusion protein Ag2478 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | signal-regulatory protein beta 1 |
别名 | SIRPB1, SIRP Beta 1, SIRP-beta-1, Signal-regulatory protein beta-1, CD172b |
计算分子量 | 43 kDa |
观测分子量 | 43 kDa |
GenBank蛋白编号 | BC025286 |
基因名称 | SIRPB1 |
Gene ID (NCBI) | 10326 |
RRID | AB_2188197 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O00241 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.
实验方案
Product Specific Protocols | |
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WB protocol for SIRP beta 1/CD172b antibody 11811-1-AP | Download protocol |
IHC protocol for SIRP beta 1/CD172b antibody 11811-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS One SNPs array karyotyping reveals a novel recurrent 20p13 amplification in primary myelofibrosis. | ||
Neuropathology Immunohistochemical characterization of microglia in Nasu-Hakola disease brains. |