SIRT1 Recombinant antibody, PBS Only
SIRT1 Recombinant Antibody for WB, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
4F14
验证数据展示
Planning an IHC experiment? We recommend our IHCeasy SIRT1 Ready-To-Use IHC Kit. SIRT1 primary antibody included.
产品信息
82790-2-PBS targets SIRT1 in WB, Indirect ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, Indirect ELISA Application Description |
Tested Reactivity | Human |
Immunogen | SIRT1 fusion protein Ag3808 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Synonyms | hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1 |
Calculated Molecular Weight | 747 aa, 82 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | BC012499 |
Gene Symbol | SIRT1 |
Gene ID (NCBI) | 23411 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96EB6 |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
背景介绍
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.