验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue, rat kidney tissue |
| Positive IHC detected in | human heart tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
15345-1-AP targets SIRT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | SIRT2 fusion protein Ag7556 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
| Synonyms | SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 37-45 kDa |
| GenBank Accession Number | BC003547 |
| Gene Symbol | SIRT2 |
| Gene ID (NCBI) | 22933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IXJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. This antibody is a rabbit polyclonal antibody raised against the N-terminal 352 residues of human SIR2 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT2 antibody 15345-1-AP | Download protocol |
| IHC protocol for SIRT2 antibody 15345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.
| ||
Glia Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons |
