SIRT2 Polyclonal antibody

SIRT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15345-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman brain tissue, mouse brain tissue, rat kidney tissue
Positive IHC detected inhuman heart tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SIRT2 Ready-To-Use IHC Kit. SIRT2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15345-1-AP targets SIRT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 SIRT2 fusion protein Ag7556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
别名 SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2
计算分子量 43 kDa
观测分子量 37-45 kDa
GenBank蛋白编号BC003547
基因名称 SIRT2
Gene ID (NCBI) 22933
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. This antibody is a rabbit polyclonal antibody raised against the N-terminal 352 residues of human SIR2 protein.

实验方案

Product Specific Protocols
WB protocol for SIRT2 antibody 15345-1-APDownload protocol
IHC protocol for SIRT2 antibody 15345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Metab

An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.

Authors - Ming He
  • KO Validated
mouse

Glia

Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons

Authors - Erik Späte
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