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SIRT2 Polyclonal antibody
SIRT2 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | rat brain tissue, human skeletal muscle tissue, mouse brain tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19655-1-AP targets SIRT2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, zebrafish, bovine, goat |
Immunogen | SIRT2 fusion protein Ag7756 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae) |
Synonyms | SIR2, Regulatory protein SIR2 homolog 2, NAD-dependent protein defatty-acylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, EC:2.3.1.286 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 37-45 kDa |
GenBank Accession Number | BC003547 |
Gene Symbol | SIRT2 |
Gene ID (NCBI) | 22933 |
RRID | AB_2878592 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8IXJ6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SIRT2 antibody 19655-1-AP | Download protocol |
IHC protocol for SIRT2 antibody 19655-1-AP | Download protocol |
IP protocol for SIRT2 antibody 19655-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Neuron Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.
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J Clin Invest SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair. | ||
Nat Commun Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling. | ||
Aging Cell SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.
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