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验证数据展示

  • WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

    WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT2 antibody included
  • IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

    IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

    WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SIRT2 Monoclonal antibody, PBS Only

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66410-1-PBS

产品说明书

CloneNo. 1D8G10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SIRT2 Ready-To-Use IHC Kit. SIRT2 primary antibody included.

产品信息

66410-1-PBS targets SIRT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SIRT2 fusion protein Ag7756 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
别名 SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2
计算分子量 43 kDa
观测分子量 37-45 kDa
GenBank蛋白编号BC003547
基因名称 SIRT2
Gene ID (NCBI) 22933
RRIDAB_2881782
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IXJ6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.