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  • Featured Product
  • KD/KO Validated

SIRT2 Monoclonal antibody, PBS Only

SIRT2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8G10

Cat no : 66410-1-PBS

Print datasheet

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

验证数据展示

  • WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

    WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT2 antibody included
  • IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

    IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

    WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SIRT2 Ready-To-Use IHC Kit. SIRT2 primary antibody included.

产品信息

66410-1-PBS targets SIRT2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SIRT2 fusion protein Ag7756 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Synonyms SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 37-45 kDa
GenBank Accession NumberBC003547
Gene Symbol SIRT2
Gene ID (NCBI) 22933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IXJ6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.