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SIRT3 Polyclonal antibody

SIRT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29838-1-AP

Print datasheet

Synonyms

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT 3, SIRT3

验证数据展示

  • WB analysis using 29838-1-AP

    WB analysis using 29838-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29838-1-AP (SIRT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 29838-1-AP

    IHC staining of human prostate cancer using 29838-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29838-1-AP (SIRT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29838-1-AP targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen SIRT3 fusion protein Ag31333 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Synonyms hSIRT3, SIR2 like protein 3, SIR2L3, SIRT 3, SIRT3
Calculated Molecular Weight 44 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC001042
Gene Symbol SIRT3
Gene ID (NCBI) 23410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

实验方案

Product Specific Protocols
WB protocol for SIRT3 antibody 29838-1-APDownload protocol
IHC protocol for SIRT3 antibody 29838-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols