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SIRT5 Monoclonal antibody, PBS Only

SIRT5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 67257-1-PBS

Print datasheet

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

验证数据展示

  • WB analysis of HeLa using 67257-1-Ig (same clone as 67257-1-PBS)

    WB analysis of HeLa using 67257-1-Ig (same clone as 67257-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

    IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

产品信息

67257-1-PBS targets SIRT5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen SIRT5 fusion protein Ag7743 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Synonyms FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5
Calculated Molecular Weight 34 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC000126
Gene Symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXA8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.