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SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28677-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 28677-1-AP

    WB analysis using 28677-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28677-1-AP (SIRT6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28677-1-AP targets SIRT6 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen SIRT6 fusion protein Ag29163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Synonyms
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC028220
Gene Symbol SIRT6
Gene ID (NCBI) 51548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIRT6 gene is located at chromosome 19p13.3 and contains 8 exons. The encoded protein has a total length of 355 amino acids. SIRT6 protein is a member of the Sirtuins, which is a class of NAD+-dependent protein deacetylases involved in stress resistance and metabolic homeostasis. SIRT6 also plays roles in various tumors as both an oncogene and tumor suppressor gene. A previous study in osteosarcoma reported that SIRT6 regulates the migration and invasion of tumors through the ERK1/2/MMP9 pathway. (PMID: 30675128)

实验方案

Product Specific Protocols
WB protocol for SIRT6 antibody 28677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols