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  • Featured Product
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SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28677-1-AP

Print datasheet

Synonyms

SIR2 like protein 6, NAD-dependent protein deacetylase sirtuin-6, hSIRT6, EC:2.4.2.-, EC:2.3.1.286

验证数据展示

  • WB analysis using 28677-1-AP

    WB analysis using 28677-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28677-1-AP (SIRT6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28677-1-AP

    WB analysis of HeLa using 28677-1-AP

    WB result of SIRT6 antibody (28677-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT6 transfected HeLa cells.

  • IHC staining of human placenta using 28677-1-AP

    IHC staining of human placenta using 28677-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28677-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 28677-1-AP

    IHC staining of rat liver using 28677-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28677-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman placenta tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28677-1-AP targets SIRT6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SIRT6 fusion protein Ag29163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Synonyms SIR2 like protein 6, NAD-dependent protein deacetylase sirtuin-6, hSIRT6, EC:2.4.2.-, EC:2.3.1.286
Calculated Molecular Weight 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC028220
Gene Symbol SIRT6
Gene ID (NCBI) 51548
RRIDAB_3669666
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6T7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIRT6 gene is located at chromosome 19p13.3 and contains 8 exons. The encoded protein has a total length of 355 amino acids. SIRT6 protein is a member of the Sirtuins, which is a class of NAD+-dependent protein deacetylases involved in stress resistance and metabolic homeostasis. SIRT6 also plays roles in various tumors as both an oncogene and tumor suppressor gene. A previous study in osteosarcoma reported that SIRT6 regulates the migration and invasion of tumors through the ERK1/2/MMP9 pathway. (PMID: 30675128)

实验方案

Product Specific Protocols
WB protocol for SIRT6 antibody 28677-1-APDownload protocol
IHC protocol for SIRT6 antibody 28677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols