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SIRT7 Polyclonal antibody

SIRT7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 29729-1-AP

Print datasheet

Synonyms

SIR2 like protein 7, SIR2L7, SIRT7

验证数据展示

  • WB analysis using 29729-1-AP

    WB analysis using 29729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29729-1-AP

    WB analysis using 29729-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 29729-1-AP

    IF Staining of HEK-293 using 29729-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIRT7 antibody (29729-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inCaco-2 cells, HeLa cells, HCT 116 cells, MCF-7 cells, rat liver cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29729-1-AP targets SIRT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human, rat
Cited Reactivityhuman, rat
Immunogen SIRT7 fusion protein Ag31794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
Synonyms SIR2 like protein 7, SIR2L7, SIRT7
Calculated Molecular Weight45KD
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_016538
Gene Symbol SIRT7
Gene ID (NCBI) 51547
RRIDAB_2918342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRC8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

实验方案

Product Specific Protocols
WB protocol for SIRT7 antibody 29729-1-APDownload protocol
IF protocol for SIRT7 antibody 29729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB,IHC

Int Urol Nephrol

SIRT7 silencing by miR-152-3p confers cell apoptosis and renal functional impairment induced by renal ischaemia/reperfusion injury

Authors - Yan Wang
View Article