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SIX1 Monoclonal antibody, PBS Only

SIX1 Monoclonal Antibody for WB,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB,IF,ELISA

Conjugate

Unconjugated

CloneNo.

2D7D1

Cat No : 67741-1-PBS

Print datasheet

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

验证数据展示

  • WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

  • WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 67741-1-Ig (same clone as 67741-1-PBS)

    IF Staining of A431 using 67741-1-Ig (same clone as 67741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SIX1 Ready-To-Use IHC Kit. SIX1 primary antibody included.

产品信息

67741-1-PBS targets SIX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Applications WB,IF,ELISA Application Description
Tested Reactivity Human, mouse, pig, rabbit, rat
Immunogen SIX1 fusion protein Ag28570 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SIX homeobox 1
Synonyms BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39
Calculated Molecular Weight 32 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC008874
Gene Symbol SIX1
Gene ID (NCBI) 6495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15475
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.