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SIX2 Monoclonal antibody, PBS Only

SIX2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, Canine, mouse, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9G11

Cat no : 66347-1-PBS

Print datasheet

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

验证数据展示

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

产品信息

66347-1-PBS targets SIX2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, Canine, mouse, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat, Canine, mouse, pig
Immunogen SIX2 fusion protein Ag2124 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name SIX homeobox 2
Synonyms Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
RRIDAB_2881727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPC8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].