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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    WB analysis using 66347-1-Ig (same clone as 66347-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 66347-1-Ig (SIX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    IHC staining of mouse embryo using 66347-1-Ig (same clone as 66347-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66347-1-Ig (SIX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66347-1-PBS in a different storage buffer formulation.

SIX2 Monoclonal antibody, PBS Only

SIX2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66347-1-PBS

产品说明书

CloneNo. 1F9G11

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat, Canine, mouse, pig

应用

WB, IHC, Indirect ELISA

别名

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66347-1-PBS targets SIX2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, Canine, mouse, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat, Canine, mouse, pig
免疫原 SIX2 fusion protein Ag2124 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SIX homeobox 2
别名 Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
计算分子量 291 aa, 32 kDa
观测分子量 37 kDa
GenBank蛋白编号BC024033
基因名称 SIX2
Gene ID (NCBI) 10736
RRIDAB_2881727
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPC8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].