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- KD/KO Validated
SKAP2 Polyclonal antibody
SKAP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, NIH/3T3 cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12926-1-AP targets SKAP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | SKAP2 fusion protein Ag3615 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | src kinase associated phosphoprotein 2 |
Synonyms | PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC036044 |
Gene Symbol | SKAP2 |
Gene ID (NCBI) | 8935 |
RRID | AB_2189317 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75563 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SKAP2 antibody 12926-1-AP | Download protocol |
IHC protocol for SKAP2 antibody 12926-1-AP | Download protocol |
IP protocol for SKAP2 antibody 12926-1-AP | Download protocol |
FC protocol for SKAP2 antibody 12926-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Host Microbe Identifying Yersinia YopH-targeted signal transduction pathways that impair neutrophil responses during in vivo murine infection. | ||
J Exp Med Skap2 is required for β2 integrin-mediated neutrophil recruitment and functions.
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Cancer Res SKAP2 Promotes Podosome Formation to Facilitate Tumor-Associated Macrophage Infiltration and Metastatic Progression. | ||
Diabetes SKAP2, a Candidate Gene for Type 1 Diabetes, Regulates β-Cell Apoptosis and Glycemic Control in Newly Diagnosed Patients.
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Elife SKAP2 is required for defense against K. pneumoniae infection and neutrophil respiratory burst. | ||