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SKAP55 Polyclonal antibody

SKAP55 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14724-1-AP

Print datasheet

Synonyms

SKAP1, SCAP1, Src kinase-associated phosphoprotein of 55 kDa, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1

验证数据展示

  • WB analysis of mouse thymus using 14724-1-AP

    WB analysis of mouse thymus using 14724-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14724-1-AP (SKAP55 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 14724-1-AP

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 14724-1-AP

    IHC staining of human tonsillitis using 14724-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14724-1-AP (SKAP55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse thymus tissue, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14724-1-AP targets SKAP55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SKAP55 fusion protein Ag6467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name src kinase associated phosphoprotein 1
Synonyms SKAP1, SCAP1, Src kinase-associated phosphoprotein of 55 kDa, Src kinase-associated phosphoprotein 1, Src family-associated phosphoprotein 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC047870
Gene Symbol SKAP55
Gene ID (NCBI) 8631
RRIDAB_10858320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKAP55 antibody 14724-1-APDownload protocol
IHC protocol for SKAP55 antibody 14724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols