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验证数据展示

  • WB analysis using 19247-1-AP

    WB analysis using 19247-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19247-1-AP (SKAP55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 19247-1-AP

    IHC staining of human tonsillitis using 19247-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19247-1-AP (SKAP55 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SKAP55 Polyclonal antibody

SKAP55 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19247-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

pp55, SCAP1, SKAP 55, SKAP1, SKAP55

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse thymus tissue, rat thymus tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19247-1-AP targets SKAP55 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SKAP55 fusion protein Ag6333 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 src kinase associated phosphoprotein 1
别名 pp55, SCAP1, SKAP 55, SKAP1, SKAP55
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC047870
基因名称 SKAP55
Gene ID (NCBI) 8631
RRIDAB_10859099
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WV1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKAP55 antibody 19247-1-APDownload protocol
IHC protocol for SKAP55 antibody 19247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols