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- KD/KO Validated
SKIV2L Polyclonal antibody
SKIV2L Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11462-1-AP targets SKIV2L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | SKIV2L fusion protein Ag1977 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | superkiller viralicidic activity 2-like (S. cerevisiae) |
| Synonyms | SKI2, HLP, Helicase SKI2W, EC:3.6.4.13, DDX13 |
| Calculated Molecular Weight | 1246 aa, 138 kDa |
| Observed Molecular Weight | 138 kDa |
| GenBank Accession Number | BC015758 |
| Gene Symbol | SKIV2L |
| Gene ID (NCBI) | 6499 |
| RRID | AB_2187472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15477 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| IHC protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| IP protocol for SKIV2L antibody 11462-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. | ||
Nucleic Acids Res Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.
| ||
Nucleic Acids Res A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans. | ||
Proc Natl Acad Sci U S A The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.
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