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SKIV2L2 Polyclonal antibody

SKIV2L2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12719-2-AP

Print datasheet

Synonyms

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

验证数据展示

  • WB analysis of HepG2 using 12719-2-AP

    WB analysis of HepG2 using 12719-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12719-2-AP

    IP experiment of HepG2 using 12719-2-AP

    IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug.

  • IHC staining of human stomach using 12719-2-AP

    IHC staining of human stomach using 12719-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12719-2-AP targets SKIV2L2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SKIV2L2 fusion protein Ag3414 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name superkiller viralicidic activity 2-like 2 (S. cerevisiae)
Synonyms Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1
Calculated Molecular Weight 1042 aa, 118 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberBC028604
Gene Symbol SKIV2L2
Gene ID (NCBI) 23517
RRIDAB_2187479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42285
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKIV2L2 antibody 12719-2-APDownload protocol
IHC protocol for SKIV2L2 antibody 12719-2-APDownload protocol
IP protocol for SKIV2L2 antibody 12719-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article
humanWB,IP

Nature

Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance

Authors - Lin Shan
View Article
humanWB

Cell Genom

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

Authors - Yahui Wang
View Article