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验证数据展示

  • WB analysis of HepG2 using 12719-2-AP

    WB analysis of HepG2 using 12719-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12719-2-AP

    IP experiment of HepG2 using 12719-2-AP

    IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:6000) with HepG2 cells lysate 1320 ug.

  • IHC staining of human stomach using 12719-2-AP

    IHC staining of human stomach using 12719-2-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12719-2-AP (SKIV2L2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 12719-2-AP

    IF Staining of mouse testis using 12719-2-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SKIV2L2 antibody (12719-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SKIV2L2 Polyclonal antibody

SKIV2L2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 12719-2-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human brain tissue, mouse testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12719-2-AP targets SKIV2L2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SKIV2L2 fusion protein Ag3414 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 superkiller viralicidic activity 2-like 2 (S. cerevisiae)
别名 Exosome RNA helicase MTR4, EC:3.6.4.13, DOB1, ATP-dependent RNA helicase SKIV2L2, ATP-dependent RNA helicase DOB1
计算分子量 1042 aa, 118 kDa
观测分子量 118 kDa
GenBank蛋白编号BC028604
基因名称 SKIV2L2
Gene ID (NCBI) 23517
RRIDAB_2187479
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42285
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SKIV2L2 antibody 12719-2-APDownload protocol
IHC protocol for SKIV2L2 antibody 12719-2-APDownload protocol
IF protocol for SKIV2L2 antibody 12719-2-APDownload protocol
IP protocol for SKIV2L2 antibody 12719-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article
humanWB,IP

Nature

Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance

Authors - Lin Shan
View Article
humanWB

Cell Genom

NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors

Authors - Yahui Wang
View Article