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SKOR2 Polyclonal antibody

SKOR2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55370-1-AP

Print datasheet

Synonyms

FUSSEL18

验证数据展示

  • WB analysis using 55370-1-AP

    WB analysis using 55370-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55370-1-AP (SKOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 55370-1-AP

    IHC staining of mouse cerebellum using 55370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKOR2 antibody included
  • IHC staining of mouse cerebellum using 55370-1-AP

    IHC staining of mouse cerebellum using 55370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKOR2 antibody included
  • IF Staining of mouse cerebellum using 55370-1-AP

    IF Staining of mouse cerebellum using 55370-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SKOR2 antibody (55370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SKOR2 Ready-To-Use IHC Kit. SKOR2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55370-1-AP targets SKOR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name functional smad suppressing element 18
Synonyms FUSSEL18
Calculated Molecular Weight 104 kDa
Observed Molecular Weight30kDa, 60 kDa
GenBank Accession NumberXM_001715219
Gene Symbol SKOR2
Gene ID (NCBI) 652991
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2VWA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKOR2 (SKI family transcriptional corepressor 2), also named FUSSEL18, is a transcriptional repressor that is highly expressed in the central nervous system (PMID: 16200078). SKOR2 was detected at 30 and 60 kD in the cerebellum. It is identified as a novel transcriptional regulator in Purkinje cells that acts upstream of Shh during cerebellum development (PMID: 21937600). Skor2 was detected in Purkinje cells in the human fetal cerebellum (PMID: 34140698).

实验方案

Product Specific Protocols
WB protocol for SKOR2 antibody 55370-1-APDownload protocol
IHC protocol for SKOR2 antibody 55370-1-APDownload protocol
IF protocol for SKOR2 antibody 55370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols