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验证数据展示

  • WB analysis using 55370-1-AP

    WB analysis using 55370-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55370-1-AP (SKOR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 55370-1-AP

    IHC staining of mouse cerebellum using 55370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKOR2 antibody included
  • IHC staining of mouse cerebellum using 55370-1-AP

    IHC staining of mouse cerebellum using 55370-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 55370-1-AP (SKOR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SKOR2 antibody included
  • IF Staining of mouse cerebellum using 55370-1-AP

    IF Staining of mouse cerebellum using 55370-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using SKOR2 antibody (55370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SKOR2 Polyclonal antibody

SKOR2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 55370-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

FUSSEL18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SKOR2 Ready-To-Use IHC Kit. SKOR2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55370-1-AP targets SKOR2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 functional smad suppressing element 18
别名 FUSSEL18
计算分子量 104 kDa
观测分子量30kDa, 60 kDa
GenBank蛋白编号XM_001715219
基因名称 SKOR2
Gene ID (NCBI) 652991
RRIDAB_3086446
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2VWA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKOR2 (SKI family transcriptional corepressor 2), also named FUSSEL18, is a transcriptional repressor that is highly expressed in the central nervous system (PMID: 16200078). SKOR2 was detected at 30 and 60 kD in the cerebellum. It is identified as a novel transcriptional regulator in Purkinje cells that acts upstream of Shh during cerebellum development (PMID: 21937600). Skor2 was detected in Purkinje cells in the human fetal cerebellum (PMID: 34140698).

实验方案

Product Specific Protocols
WB protocol for SKOR2 antibody 55370-1-APDownload protocol
IHC protocol for SKOR2 antibody 55370-1-APDownload protocol
IF protocol for SKOR2 antibody 55370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols