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SLAMF6 Polyclonal antibody

SLAMF6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22711-1-AP

Print datasheet

Synonyms

Activating NK receptor, CD352, KALI, KALIb, Ly108, NK T B antigen, NTB A, NTBA, SF2000, SLAM family member 6, SLAMF6

验证数据展示

  • IHC staining of human colon cancer using 22711-1-AP

    IHC staining of human colon cancer using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Daudi using 22711-1-AP

    WB analysis of Daudi using 22711-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 22711-1-AP (SLAMF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 22711-1-AP

    IHC staining of human colon cancer using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 22711-1-AP

    IHC staining of human tonsillitis using 22711-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDaudi cells
Positive IHC detected inhuman colon cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22711-1-AP targets SLAMF6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SLAMF6 fusion protein Ag18517 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLAM family member 6
Synonyms Activating NK receptor, CD352, KALI, KALIb, Ly108, NK T B antigen, NTB A, NTBA, SF2000, SLAM family member 6, SLAMF6
Calculated Molecular Weight 332 aa, 37 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC114495
Gene Symbol SLAMF6
Gene ID (NCBI) 114836
RRIDAB_2879154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DU3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).

实验方案

Product Specific Protocols
WB protocol for SLAMF6 antibody 22711-1-APDownload protocol
IHC protocol for SLAMF6 antibody 22711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols