验证数据展示
经过测试的应用
Positive WB detected in | IM-9 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84254-1-RR targets SLAMF7/CD319 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SLAM family member 7 |
Synonyms | SLAMF7, FOAP-12, CS1, CRACC, CD319 |
Calculated Molecular Weight | 335 aa, 37 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC027867 |
Gene Symbol | SLAMF7 |
Gene ID (NCBI) | 57823 |
RRID | AB_3671802 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9NQ25 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signaling lymphocyte activation molecule (SLAM) family receptors is a group of type I transmembrane receptors belonging to the immunoglobulin superfamily (PMID: 37251372). The SLAM family contains nine members that contain an extracellular segment comprising two or four Ig-like domains (V-like variable and C2-like constant), a transmembrane region and a cytoplasmic tail (PMID: 29662641). They are involved in various physiological and pathological processes, including the regulation of immunological responses and the route of viral entry. SLAMF7, also known as CS1, CRACC or CD319, is expressed on NK cells, CD8+ T lymphocytes, B lymphocytes, and mature dendritic cells (PMID: 28861320). It exerts both activating and inhibitory effects depending on the expression status of SAP or EAT-2. SLAMF7 is overexpressed in multiple myeloma and makes it a target for immunotherapy (PMID: 28861320). The native molecular weight of SLAMF7 is 37 kDa and the apparent molecular mass shifts due to glycosylation (PMID: 11698418; 36381324).
实验方案
Product Specific Protocols | |
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WB protocol for SLAMF7/CD319 antibody 84254-1-RR | Download protocol |
IHC protocol for SLAMF7/CD319 antibody 84254-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |