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SLC10A6 Polyclonal antibody

SLC10A6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27938-1-AP

Print datasheet

Synonyms

SLC10A6, SOAT

验证数据展示

  • WB analysis of mouse liver using 27938-1-AP

    WB analysis of mouse liver using 27938-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 27938-1-AP (SLC10A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 27938-1-AP

    IHC staining of mouse heart using 27938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27938-1-AP (SLC10A6 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse heart tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27938-1-AP targets SLC10A6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen SLC10A6 fusion protein Ag16609 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 10 (sodium/bile acid cotransporter family), member 6
Synonyms SLC10A6, SOAT
Calculated Molecular Weight 377 aa, 41 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC107051
Gene Symbol SLC10A6
Gene ID (NCBI) 345274
RRIDAB_2881014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KNW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC10A6, also named as SOAT, belongs to the SlC10 sodium-dependent bile acid (BA) transporter family. SLC10A6 is a transporter of steroid sulfates associated with spermatogenesis and fertility. It has been shown that SLC10A6 might be a new anti-proliferative breast cancer target as the SLC10A6 inhibition can affect the proliferation of breast cancer cells. SLC10A6 also plays a key role in hepatic inflammation. 27938-1-AP antibody detects the 70 kDa glycosylated form in SDS-PAGE. (PMID: 26510996, 30186172, 28893621)

实验方案

Product Specific Protocols
WB protocol for SLC10A6 antibody 27938-1-APDownload protocol
IHC protocol for SLC10A6 antibody 27938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols