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SLC12A4 Polyclonal antibody

SLC12A4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15927-1-AP

Print datasheet

Synonyms

Erythroid K Cl cotransporter 1, hKCC1, KCC1, SLC12A4

验证数据展示

  • IHC staining of human liver cancer using 15927-1-AP

    IHC staining of human liver cancer using 15927-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15927-1-AP (SLC12A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 15927-1-AP

    WB analysis of HeLa using 15927-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15927-1-AP (SLC12A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15927-1-AP

    IP experiment of HeLa using 15927-1-AP

    IP result of anti-SLC12A4 (IP:15927-1-AP, 4ug; Detection:15927-1-AP 1:500) with HeLa cells lysate 3200ug.

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15927-1-AP targets SLC12A4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC12A4 fusion protein Ag8762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 12 (potassium/chloride transporters), member 4
Synonyms Erythroid K Cl cotransporter 1, hKCC1, KCC1, SLC12A4
Calculated Molecular Weight 1085 aa, 121 kDa
Observed Molecular Weight 121 kDa
GenBank Accession NumberBC021193
Gene Symbol SLC12A4
Gene ID (NCBI) 6560
RRIDAB_2188666
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UP95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC12A4 antibody 15927-1-APDownload protocol
IHC protocol for SLC12A4 antibody 15927-1-APDownload protocol
IP protocol for SLC12A4 antibody 15927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols