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验证数据展示

  • IF Staining of U2OS using 83225-2-RR (same clone as 83225-2-PBS)

    IF Staining of U2OS using 83225-2-RR (same clone as 83225-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC12A8 antibody (83225-2-RR, Clone: 240082B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR (same clone as 83225-2-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 83225-2-RR (same clone as 83225-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83225-2-RR (SLC12A8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83225-2-RR (same clone as 83225-2-PBS)

    FC experiment of MCF-7 using 83225-2-RR (same clone as 83225-2-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SLC12A8 Recombinant antibody (83225-2-RR, Clone:240082B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83225-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00171-1

    Cytometric bead array standard curve of MP00171-1

    Cytometric bead array standard curve of MP00171-1, SLC12A8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83225-1-PBS. Detection antibody: 83225-2-PBS. Standard: Ag34354. Range: 5-40 ng/mL.

  • Affinity and Kinetic Characterization of 83225-2-RR

    Affinity and Kinetic Characterization of 83225-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83225-2-RR against Human SLC12A8 were performed. The affinity constant is 3.03 nM.

SLC12A8 Recombinant antibody, PBS Only (Detector)

SLC12A8 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83225-2-PBS

产品说明书

CloneNo. 240082B11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

CCC9, Cation-chloride cotransporter 9, 240082B11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83225-2-PBS targets SLC12A8 as part of a matched antibody pair:

MP00171-1: 83225-1-PBS capture and 83225-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC12A8 fusion protein Ag34354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 12 (potassium/chloride transporters), member 8
别名 CCC9, Cation-chloride cotransporter 9, 240082B11
计算分子量 714 aa, 80 kDa
GenBank蛋白编号BC020506
基因名称 SLC12A8
Gene ID (NCBI) 84561
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDA0AV02
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C