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验证数据展示

  • WB analysis of HEK-293 using 20302-1-AP

    WB analysis of HEK-293 using 20302-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20302-1-AP (SLC13A4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 20302-1-AP

    IF Staining of HEK-293 using 20302-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20302-1-AP (SLC13A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SLC13A4 Polyclonal antibody

SLC13A4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20302-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

SUT1, SUT 1, NaS2, Na(+)/sulfate cotransporter SUT-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20302-1-AP targets SLC13A4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SLC13A4 fusion protein Ag14116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 13 (sodium/sulfate symporters), member 4
Synonyms SUT1, SUT 1, NaS2, Na(+)/sulfate cotransporter SUT-1
Calculated Molecular Weight 626 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC030689
Gene Symbol SLC13A4
Gene ID (NCBI) 26266
RRIDAB_10793722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKG4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC13A4 (also known as SUT-1 or NaS2) belongs to the family of Na+-coupled anion transporters and mediates sulfate reabsorption in the high endothelial venules (HEV). Sulfate is an obligate nutrient for fetal development. Highly expressed in placenta, SLC13A4 has been proposed to play important physiological roles in maintaining high maternal serum sulfate levels during pregnancy and mediating sulfate supply to the fetus.

实验方案

Product Specific Protocols
WB protocol for SLC13A4 antibody 20302-1-APDownload protocol
IF protocol for SLC13A4 antibody 20302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Biol Reprod

Placental, renal, and ileal sulfate transporter gene expression in mouse gestation.

Authors - Dawson Paul A PA
View Article
mouseIHC

EBioMedicine

Postnatal N-acetylcysteine administration rescues impaired social behaviors and neurogenesis in Slc13a4 haploinsufficient mice.

Authors - Zhe Zhang
View Article