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SLC16A11 Recombinant antibody

SLC16A11 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240081H5

Cat no : 83239-5-RR

Print datasheet

Synonyms

Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5

验证数据展示

  • WB analysis using 83239-5-RR

    WB analysis using 83239-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83239-5-RR (SLC16A11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83239-5-RR

    FC experiment of A549 using 83239-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC16A11 Recombinant antibody (83239-5-RR, Clone:240081H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83239-5-RR

    Affinity and Kinetic Characterization of 83239-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83239-5-RR against Human SLC16A11 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue, mouse stomach tissue
Positive FC (Intra) detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83239-5-RR targets SLC16A11 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC16A11 fusion protein Ag24920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 16, member 11 (monocarboxylic acid transporter 11)
Synonyms Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5
Calculated Molecular Weight 471 aa, 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC093860
Gene Symbol SLC16A11
Gene ID (NCBI) 162515
RRIDAB_3670915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8NCK7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC16A11 antibody 83239-5-RRDownload protocol
FC protocol for SLC16A11 antibody 83239-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols