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验证数据展示

  • WB analysis using 83239-5-RR

    WB analysis using 83239-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83239-5-RR (SLC16A11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83239-5-RR

    FC experiment of A549 using 83239-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC16A11 Recombinant antibody (83239-5-RR, Clone:240081H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83239-5-RR

    Affinity and Kinetic Characterization of 83239-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83239-5-RR against Human SLC16A11 were performed. The affinity constant is below 1 pM.

SLC16A11 Recombinant antibody

SLC16A11 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83239-5-RR

产品说明书

CloneNo. 240081H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse brain tissue, mouse stomach tissue
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83239-5-RR targets SLC16A11 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC16A11 fusion protein Ag24920 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 16, member 11 (monocarboxylic acid transporter 11)
别名 Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5
计算分子量 471 aa, 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC093860
基因名称 SLC16A11
Gene ID (NCBI) 162515
RRIDAB_3670915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8NCK7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC16A11 antibody 83239-5-RRDownload protocol
FC protocol for SLC16A11 antibody 83239-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols