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SLC16A5 Polyclonal antibody

SLC16A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12120-1-AP

Print datasheet

Synonyms

MCT 5, MCT 6, MCT5, MCT6, Monocarboxylate transporter 5, Monocarboxylate transporter 6, SLC16A5

验证数据展示

  • WB analysis of mouse skeletal muscle using 12120-1-AP

    WB analysis of mouse skeletal muscle using 12120-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12120-1-AP (SLC16A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12120-1-AP

    IHC staining of human breast cancer using 12120-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12120-1-AP (SLC16A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human placenta tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12120-1-AP targets SLC16A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC16A5 fusion protein Ag2762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 16, member 5 (monocarboxylic acid transporter 6)
Synonyms MCT 5, MCT 6, MCT5, MCT6, Monocarboxylate transporter 5, Monocarboxylate transporter 6, SLC16A5
Calculated Molecular Weight 505 aa, 55 kDa
Observed Molecular Weight50-55 kDa
GenBank Accession NumberBC009684
Gene Symbol SLC16A5
Gene ID (NCBI) 9121
RRIDAB_2877827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15375
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Monocarboxylate transporter 5 (SLC16A5), also named as MCT6, is a member of the MCT family. SLC16A5 is a proton-linked monocarboxylate transporter that catalyzes the rapid transport across the plasma membrane of many monocarboxylates and it can transport various drugs. It has been reported that SLC16A5 mutation may be a novel genetic risk factor for cisplatin-induced ototoxic (CIO) in testicular cancer patients. There are some isoforms of SLC16A5 among 50-55 kDa. (PMID: 16174808, 28448657)

实验方案

Product Specific Protocols
WB protocol for SLC16A5 antibody 12120-1-APDownload protocol
IHC protocol for SLC16A5 antibody 12120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols