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验证数据展示

  • WB analysis using 26731-1-AP

    WB analysis using 26731-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26731-1-AP (SLC17A9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 26731-1-AP

    IP experiment of mouse brain using 26731-1-AP

    IP result of anti-SLC17A9 (IP:26731-1-AP, 4ug; Detection:26731-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

  • IHC staining of human skin using 26731-1-AP

    IHC staining of human skin using 26731-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 26731-1-AP

    IHC staining of human skin using 26731-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 26731-1-AP (SLC17A9 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of human pancreas using 26731-1-AP

    IF Staining of human pancreas using 26731-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human pancreas using 26731-1-AP

    IF Staining of human pancreas using 26731-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using 26731-1-AP (SLC17A9 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

SLC17A9 Polyclonal antibody

SLC17A9 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 26731-1-AP

Print datasheet

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Synonyms

C20orf59, SLC17A9, Vesicular nucleotide transporter, VNUT

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue, Caco-2 cells, THP-1 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26731-1-AP targets SLC17A9 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SLC17A9 fusion protein Ag24970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 17, member 9
Synonyms C20orf59, SLC17A9, Vesicular nucleotide transporter, VNUT
Calculated Molecular Weight 436 aa, 47 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC025312
Gene Symbol SLC17A9
Gene ID (NCBI) 63910
RRIDAB_2880617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC17A9 antibody 26731-1-APDownload protocol
IHC protocol for SLC17A9 antibody 26731-1-APDownload protocol
IF protocol for SLC17A9 antibody 26731-1-APDownload protocol
IP protocol for SLC17A9 antibody 26731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,WB

iScience

SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma

Authors - Weiquan Li
  • KD Validated
View Article
humanIHC

bioRxiv

Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue

Authors - Su Min Hong
View Article
human,mouseIHC,IF

iScience

A purinergic mechanism underlying metformin regulation of hyperglycemia

Authors - Jared Senfeld
View Article
humanWB

Oncol Lett

SLC17A9 expression levels in a pan‑cancer panel and validation of the role of SLC17A9 as a novel prognostic biomarker for osteosarcoma

Authors - Junqing Li
  • KD Validated
View Article