验证数据展示
经过测试的应用
Positive WB detected in | rat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue, Caco-2 cells, THP-1 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human pancreas tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26731-1-AP targets SLC17A9 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | SLC17A9 fusion protein Ag24970 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 17, member 9 |
别名 | C20orf59, SLC17A9, Vesicular nucleotide transporter, VNUT |
计算分子量 | 436 aa, 47 kDa |
观测分子量 | 68-70 kDa |
GenBank蛋白编号 | BC025312 |
基因名称 | SLC17A9 |
Gene ID (NCBI) | 63910 |
RRID | AB_2880617 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BYT1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SLC17A9 antibody 26731-1-AP | Download protocol |
IHC protocol for SLC17A9 antibody 26731-1-AP | Download protocol |
IF protocol for SLC17A9 antibody 26731-1-AP | Download protocol |
IP protocol for SLC17A9 antibody 26731-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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iScience SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma
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bioRxiv Optimization of protocols for immunohistochemical assessment of enteric nervous system in formalin fixed human tissue | ||
Oncol Lett SLC17A9 expression levels in a pan‑cancer panel and validation of the role of SLC17A9 as a novel prognostic biomarker for osteosarcoma
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