验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, LNCaP cells |
| Positive IHC detected in | human placenta tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
25958-1-AP targets SLC19A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SLC19A1 fusion protein Ag22921 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 19 (folate transporter), member 1 |
| 别名 | CHMD, FLOT1, Folate transporter 1, FOLT, IFC 1, IFC1, Intestinal folate carrier 1, Placental folate transporter, Reduced folate carrier protein, REFC, RFC, RFC1, SLC19A1 |
| 计算分子量 | 591 aa, 65 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC003068 |
| 基因名称 | SLC19A1 |
| Gene ID (NCBI) | 6573 |
| RRID | AB_2880310 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P41440 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC19A1, also named as RFC (reduced folate carrier), is an anionic exchanger that involved in the regulation of intracellular concentrations of folate in exchange for anions. The predicted MW of SLC19A1 is 65 kDa and 25958-1-AP antibody recognizes the 46 kDa protein which is similar to papers. The 38-40 kDa minor forms and 58 kDa form have also been reported that could be alternate splicing, post-transcriptional modification or the role of accessory proteins. (PMID: 17404734, 9111015)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC19A1 antibody 25958-1-AP | Download protocol |
| IHC protocol for SLC19A1 antibody 25958-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest An alternatively spliced STING isoform localizes in the cytoplasmic membrane and directly senses extracellular cGAMP
| ||
Cell Chem Biol A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP. | ||
Sci Adv 2'3'-cGAMP interactome identifies 2'3'-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity |
