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SLC19A3 Polyclonal antibody

SLC19A3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13407-1-AP

Print datasheet

Synonyms

SLC19A3, Thiamine transporter 2, ThTr 2, THTR2

验证数据展示

  • WB analysis using 13407-1-AP

    WB analysis using 13407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse liver using 13407-1-AP

    IP experiment of mouse liver using 13407-1-AP

    IP result of anti-SLC19A3 (IP:13407-1-AP, 4ug; Detection:13407-1-AP 1:600) with mouse liver tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse kidney tissue, human placenta tissue, rat heart tissue, 3T3-L1 cells, rat liver tissue
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13407-1-AP targets SLC19A3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SLC19A3 fusion protein Ag4053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 19, member 3
Synonyms SLC19A3, Thiamine transporter 2, ThTr 2, THTR2
Calculated Molecular Weight 496 aa, 56 kDa
Observed Molecular Weight 63-70 kDa
GenBank Accession NumberBC032014
Gene Symbol SLC19A3
Gene ID (NCBI) 80704
RRIDAB_2187997
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZV2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC19A3 antibody 13407-1-APDownload protocol
IP protocol for SLC19A3 antibody 13407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Sci Transl Med

CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.

Authors - Sara Picó
View Article
humanWB

Nat Commun

Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia

Authors - Alaullah Sheikh
View Article
humanWB

Front Cell Dev Biol

Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.

Authors - Jane L Tarry-Adkins
View Article
humanWB,IF

J Nutr Biochem

Up-regulation of vitamin B1 homeostasis genes in breast cancer.

Authors - Zastre Jason A JA
View Article
humanWB

J Biol Chem

Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.

Authors - Subrata Sabui
View Article
mouseWB

Front Pharmacol

Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice.

Authors - Hong Xu
View Article