EAAT2 Polyclonal antibody

EAAT2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 22515-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IP, ELISA and More (2)

EAAT2, GLT 1, GLT1, SLC1A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy EAAT2/SLC1A2 Ready-To-Use IHC Kit. EAAT2/SLC1A2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22515-1-AP targets EAAT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 EAAT2 fusion protein Ag18247 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 1 (glial high affinity glutamate transporter), member 2
别名 EAAT2, GLT 1, GLT1, SLC1A2
计算分子量 574 aa, 62 kDa
观测分子量 60-70 kDa, 140 kDa
GenBank蛋白编号BC132768
基因名称 EAAT2
Gene ID (NCBI) 6506
RRIDAB_2879112
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE. (PMID: 22114258)

实验方案

Product Specific Protocols
WB protocol for EAAT2 antibody 22515-1-APDownload protocol
IP protocol for EAAT2 antibody 22515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Theranostics

Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity.

Authors - Longze Sha
mouseIF

Oxid Med Cell Longev

Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression.

Authors - Di Zhang
mouseWB

Aging Cell

HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity.

Authors - Chunjiang Yu
pigWB

Neural Regen Res

Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity

Authors - Ke-Xin Li
mouseWB

Biochem Pharmacol

Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability

Authors - Yu Zhou
mouseWB

Oxid Med Cell Longev

Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson's Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota

Authors - Xiaoting Zhou
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