验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22515-1-AP targets EAAT2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig |
免疫原 | EAAT2 fusion protein Ag18247 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | solute carrier family 1 (glial high affinity glutamate transporter), member 2 |
别名 | EAAT2, GLT 1, GLT1, SLC1A2 |
计算分子量 | 574 aa, 62 kDa |
观测分子量 | 60-70 kDa, 140 kDa |
GenBank蛋白编号 | BC132768 |
基因名称 | EAAT2 |
Gene ID (NCBI) | 6506 |
RRID | AB_2879112 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P43004 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE. (PMID: 22114258)
实验方案
Product Specific Protocols | |
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WB protocol for EAAT2 antibody 22515-1-AP | Download protocol |
IP protocol for EAAT2 antibody 22515-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics Hsp90 inhibitor HSP990 in very low dose upregulates EAAT2 and exerts potent antiepileptic activity. | ||
Oxid Med Cell Longev Photobiomodulation Therapy Ameliorates Glutamatergic Dysfunction in Mice with Chronic Unpredictable Mild Stress-Induced Depression. | ||
Aging Cell HIV and drug abuse mediate astrocyte senescence in a β-catenin-dependent manner leading to neuronal toxicity. | ||
Neural Regen Res Astrocyte-neuron communication mediated by the Notch signaling pathway: focusing on glutamate transport and synaptic plasticity | ||
Biochem Pharmacol Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability | ||
Oxid Med Cell Longev Neuroprotective Effect of Ceftriaxone on MPTP-Induced Parkinson's Disease Mouse Model by Regulating Inflammation and Intestinal Microbiota |